Simultaneous determination of raloxifene and tamoxifen using dispersive micro solid-phase extraction and HPLC method in real water, human hair and serum samples

A sensitive determination procedure was presented for two anticancer drugs (tamoxifen and raloxifene) in real water biological samples based on magnetic dispersive micro solid-phase extraction–high-performance liquid chromatography with an ultraviolet detector. Fe3O4 NPs@SiO2@TiO2 nanocomposite synthesised as a sorbent by the sol-gel technique. The effective parameters were optimised experimental design, including screening optimisation steps. Under optimum conditions, displayed wide linear ranges of 5.3–735.0 7.0–806.0 ng mL?1 R-squared 0.9916 0.9908, limit detections 1.6 2.1 raloxifene tamoxifen determination, respectively. intra-day relative standard deviations 3.76 3.59% three times measurements, preconcentration factors concentration 20 506.1 546.7, method applied to analyse well water, tap hair determine simultaneously recoveries between 88.5–96.4 lower than 8.4%. method’s advantages measurement included easy operation, low desorption solvent consumption, simple preparation separation, suitable analysis time.